Murine RNase inhibitor
Description
Murine RNase inhibitor is a recombinant murine RNase inhibitor expressed and purified from E.coli. It binds to RNase A, B or C in a 1:1 ratio through non-covalent bonding, thereby inhibiting the activity of the three enzymes and protecting RNA from degradation. However, It is noteffective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. Murine RNase inhibitor was tested by RT-PCR, RT-qPCR and IVT mRNA, and was compatible with various commercial Reverse transcriptases, DNA polymerases and RNA polymerases.
Compared to human RNase inhibitors, murine RNase inhibitor does not contain two cysteines that are highly sensitive to oxidation which causes inactivation of the inhibitor. That making it stable at low concentrations of DTT (less than 1 mM). This feature makes it suitable for use in reactions where high concentrations of DTT is adverse to the reaction (eg., Real-time RT-PCR)
Chemical structure
Speficification
|
Test items |
Specifications |
|
Enzyme Activity |
40±2U/μL |
|
Endonuclease Activity |
None detected |
|
Protein Purity |
≥95% |
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Exonulease Activity |
None detected |
|
Nicking Activity |
None detected |
|
Nickase Activity |
None detected |
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E.coli DNA |
≤0.1pg/40U |
Applications
Synthesis of the first strand of cDNA, RT-PCR, RT-qPCR, etc.
Protects RNA from degradation during in vitro transcription/translation (e.g., in vitro viral replication system).
Inhibition of RNase activity during RNA separation and purification.
Shipping and Storage
Transportation: Ice packs
Storage Conditions: Store at -20 ℃,
Recommended re-test date: 2 years
