DNase I (Rnase Free) (2u/ul)
Cat No: HC4007B
DNase I is an endonuclease that can digest single-or double-stranded DNA. It can hydrolyze phosphodiester bonds to produce mono-and oligodeoxynucleotides containing a 5'-phosphate group and a 3'-OH group. The optimal working pH range of DNase I is 7-8. The activity of DNase I depends on Ca2+ and can be activated by divalent metal ions such as CO2, Mn2+, Zn2+, etc. In the presence of Mg2+, DNase I can randomly cleave any site of double-stranded DNA; While in the presence of Mn2+, DNase I can cleave DNA double-stranded at the same site, forming blunt ends or sticky ends with 1-2 nucleotides protruding. It can be used for the processing of various RNA samples.
Components
|
Name |
1KU |
5KU |
|
Recombinant DNaseI (RNase-free) |
500 μL |
5 ×500 μL |
|
DNase I Reaction Buffer (10×) |
1 mL |
5 × 1mL |
Storage conditions
This product should be stored at -25~-15℃ for 2 years. Please avoid repeated freeze-thaw.
Instructions
Applied to the removal of DNA from RNA samples for reference only.
1. Please use RNase-free centrifuge tubes and pipette tips to prepare the following reaction system:
|
Components |
Volume (μL) |
|
DNase I Reaction Buffer (10×) |
1 |
|
Recombinant DNasel (RNase-free) |
1 |
|
RNA |
X |
|
RNase-free ddH2O |
Up to 10 |
2. The reaction conditions are as follows: 37℃, after 15-30 mins, add a final concentration of 2.5 mM EDTA solution and mix well, then 65℃ for 10 mins. The processed template can be used for subsequent RT-PCR or RT-qPCR experiments, etc.
Notes
1. DNase l is sensitive to physical denaturation; When mixing, gently reverse the test tube and shake well, do not shake vigorously.
2. The enzyme should be stored in an ice box or on an ice bath when used, and should be store at -20℃ immediately after use.
3. This product is for research use only.
4. Please operate with lab coats and disposable gloves, for your safety.
