Bst DNA Polymerase
Description
Bst DNA Polymerase V2 is a homology of Bacillus Stearothermophilus DNA polym- erase 1(Bst DNA polymerase V1) and was designed by computer simulation. Bst DNA polymerase V2 has 5´→3´ polymerase activity and strong chain substitution activity, but does not have 5´→3´ exonuclease activity. Compared with wild-type Bst DNA polymerase, the enzyme can effectively increase the amplification speed, yield, salt tolerance and thermal stability, etc.
Store Buffer: 10 mM Tris-HCl pH 7.1，50 mM KCl，0.1 mM EDTA，1 mM DTT，0.1% Triton X-100，50% Glycerin
Chemical structure
Components
|
Component |
400U |
800U |
40000u |
|
Bst DNA Polymerase |
0.1 mL |
1 mL |
1 0mL |
|
Bst Buffer |
1.5 mL |
2×1.5 mL |
3×10 mL |
|
MgSO4 (100 mM) |
1.5 mL |
2×1.5 mL |
2×10 mL |
Specification
| Test items | Specification | Results |
| Protein Purity | ≥95% | Pass |
| Endonuclease Activity | Not detected | Pass |
| Exonuclease Activity | Not detected | Pass |
| Rnase Activity | Not detected | Pass |
| Non specificity | Not detected | Pass |
Applications
Isothermal amplification.
DNA sequencing with high GC content
Rapid Sequencing from nanogram amounts of DNA template.
Shipping and Storage
Transportation: Ice packs
Storage Conditions: Storage at -25 ～ -15℃.
Recommended re-test date: 18 months
Cautions
Avoid repeated freezing and thawing. The product is valid for 18 months.
