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RT-LAMP Fluorescent master mix (Lyophilized beads) HYB414 Featured Image
  • RT-LAMP Fluorescent master mix (Lyophilized beads) HYB414

RT-LAMP Fluorescent (Lyophilized beads)


RT-LAMP Fluorescent Master Mix (Lyophilized beads) contains reaction buffer, Bst DNA polymerase and heat-resistant reverse transcriptase, lyophilized protectant, and fluorescent dye.
The product is in the form of lyophilized beads, and only primers and templates need to be added for use. This reagent has high amplification efficiency and sensitivity

Cat No: HYB414

Package: 96RXN/960RXN/9600RXN

 

Product Description

Product detail

Product Tags

Reagent Component

RT-LAMP Fluorescent Master Mix (Lyophilized Powder)

 

Usage

For DNA or RNA isothermal amplification

 

Storage Conditions

Transport and store at 2~8°C.

Shelf life: 12 months.

 

Protocol

1.  Take out the corresponding number of lyophilized beads according to the number of tests.

2.  Reaction system preparation

 

Component

Dosage

RT-LAMP  Fluorescent  Master  Mix

(Lyophilized Powder)

2 beads

Fluorescent dye

x μL

10 × Primer Mix a

5 μL

Templates DNA/ RNA b

n μL

Total

50 μL

 

Notes:

1)  a. 10×Primer Mix : 16 μM FIP/BIP, 2 μM F3/B3, 4 μM Loop F/B;

2)  b. The nucleic acid template is recommended to be dissolved in DEPC water.

Hyasen Biotechnology Co., Ltd

3)  Reaction program

Temperature

Time

Cycles

65℃

60s a

30

Notes:

1. Collect fluorescent sign

 

Notes

1.  The reaction temperature can be optimized  between 62°C and 68°C according to the primer conditions;

2.  The experiment should standardize the operation, including the preparation of the reaction system,sample processing and dosing;

3.  To avoid contamination, it is recommended to prepare the reaction system in an ultra-clean table and add templates to the fume hood in other rooms to avoid false positive interference.

 


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  • Reagent Component

    RT-LAMP Fluorescent Master Mix (Lyophilized Powder)

     

    Usage

    For DNA or RNA isothermal amplification

     

    Storage Conditions

    Transport and store at 2~8°C.

    Shelf life: 12 months.

     

    Protocol

    1.  Take out the corresponding number of lyophilized beads according to the number of tests.

    2.  Reaction system preparation

     

    Component

    Dosage

    RT-LAMP  Fluorescent  Master  Mix

    (Lyophilized Powder)

    2 beads

    Fluorescent dye

    x μL

    10 × Primer Mix a

    5 μL

    Templates DNA/ RNA b

    n μL

    Total

    50 μL

     

    Notes:

    1)  a. 10×Primer Mix : 16 μM FIP/BIP, 2 μM F3/B3, 4 μM Loop F/B;

    2)  b. The nucleic acid template is recommended to be dissolved in DEPC water.

    Hyasen Biotechnology Co., Ltd

    3)  Reaction program

    Temperature

    Time

    Cycles

    65℃

    60s a

    30

    Notes:

    1. Collect fluorescent sign

     

    Notes

    1.  The reaction temperature can be optimized  between 62°C and 68°C according to the primer conditions;

    2.  The experiment should standardize the operation, including the preparation of the reaction system,sample processing and dosing;

    3.  To avoid contamination, it is recommended to prepare the reaction system in an ultra-clean table and add templates to the fume hood in other rooms to avoid false positive interference.

     

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