RT-LAMP Fluorescent (Lyophilized beads)
Reagent Component
RT-LAMP Fluorescent Master Mix (Lyophilized Powder)
Usage
For DNA or RNA isothermal amplification
Storage Conditions
Transport and store at 2~8°C.
Shelf life: 12 months.
Protocol
1. Take out the corresponding number of lyophilized beads according to the number of tests.
2. Reaction system preparation
|
Component |
Dosage |
|
RT-LAMP Fluorescent Master Mix (Lyophilized Powder) |
2 beads |
|
Fluorescent dye |
x μL |
|
10 × Primer Mix a |
5 μL |
|
Templates DNA/ RNA b |
n μL |
|
Total |
50 μL |
Notes:
1) a. 10×Primer Mix : 16 μM FIP/BIP, 2 μM F3/B3, 4 μM Loop F/B;
2) b. The nucleic acid template is recommended to be dissolved in DEPC water.
Hyasen Biotechnology Co., Ltd
3) Reaction program
|
Temperature |
Time |
Cycles |
|
65℃ |
60s a |
30 |
Notes:
1. Collect fluorescent sign
Notes
1. The reaction temperature can be optimized between 62°C and 68°C according to the primer conditions;
2. The experiment should standardize the operation, including the preparation of the reaction system,sample processing and dosing;
3. To avoid contamination, it is recommended to prepare the reaction system in an ultra-clean table and add templates to the fume hood in other rooms to avoid false positive interference.
Reagent Component
RT-LAMP Fluorescent Master Mix (Lyophilized Powder)
Usage
For DNA or RNA isothermal amplification
Storage Conditions
Transport and store at 2~8°C.
Shelf life: 12 months.
Protocol
1. Take out the corresponding number of lyophilized beads according to the number of tests.
2. Reaction system preparation
|
Component |
Dosage |
|
RT-LAMP Fluorescent Master Mix (Lyophilized Powder) |
2 beads |
|
Fluorescent dye |
x μL |
|
10 × Primer Mix a |
5 μL |
|
Templates DNA/ RNA b |
n μL |
|
Total |
50 μL |
Notes:
1) a. 10×Primer Mix : 16 μM FIP/BIP, 2 μM F3/B3, 4 μM Loop F/B;
2) b. The nucleic acid template is recommended to be dissolved in DEPC water.
Hyasen Biotechnology Co., Ltd
3) Reaction program
|
Temperature |
Time |
Cycles |
|
65℃ |
60s a |
30 |
Notes:
1. Collect fluorescent sign
Notes
1. The reaction temperature can be optimized between 62°C and 68°C according to the primer conditions;
2. The experiment should standardize the operation, including the preparation of the reaction system,sample processing and dosing;
3. To avoid contamination, it is recommended to prepare the reaction system in an ultra-clean table and add templates to the fume hood in other rooms to avoid false positive interference.
