One Step RT-qPCR Kit

One Step RT-PCR Kit is specially designed for end-point PCR detection using RNA as template (such as RNA virus). Using gene-specific primers (GSP), reverse transcription and PCR reactions are completed in one tube, no additional tube opening/pipetting operations are required, which greatly improves detection throughput and reduces the risk of contamination. IntegrateReverse Transcriptase and the superior performance of DNA Polymerase designed for long fragment amplification, and cooperate with the optimized buffer system One Step RT-PCR Kit to amplify fragments up to 10 kb or more. The kit is provided in a convenient Master Mix format. 2 × One Step Mix contains optimized buffer system and dNTP; One Step Enzyme Mix contains optimized ratio of Reverse Transcriptase, RNase inhibitor and DNA Polymerase.