Lyo-Ready Taqman Multiplex qPCR Mix

Lyo-Ready Taqman Multiplex qPCR Mix is a new generation antibody modified Taq DNA Polymerase fluroescence quantitative qPCR can be used in lyophilization reagent. It suitable for FAM/HEX/ TET/ JOE/ROX etc. Double-labled probes (Also suitable for Molecular Beacon probes). The reagent contains hotstart Fast Taq DNA Polymerase, dNTP etc. Component which can be free-dried directly on the machine.

Cat No: HYA421

Package: 100RXN/1000RXN/10000RXN

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Product Description

Product detail

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Components

Lyo-Ready TaqMan qPCR Mix

Storage Conditions

This product should be stored at -25~-15℃ away from light for 1 year.

Instructions

1.Reaction system

Components	Volume (μL)	Final concentration
Enzyme Mix	20	-
Primer Primer (10μM)	x	0.1-0.5μM
Probe Primer (10μM)	x	50-250nM
DNA Tamplate	1-25	-
dd H₂O	up to 25	-

2.Reaction program

Cycle step	Temp.	Time	Cycles
Initial denaturation	95℃	5mins	1
Amplification reaction	95℃	15sec	45
Annealing/Extension	60℃	30sec	45

Notes: In special cases the annealing/extension temperature can be adjusted according to the primer Tm value, 60°C is recommended.

Lyophilization system preparation

Components	Volume (μL)	Final concentration
Lyo-ready Taqman qPCR mix	20	1X
-50℃	x	0.1-0.5μM
-50℃	x	50-250nM
Total Volume	21-25	-

Lyophilization Process

Procedure	Temp.	Set time	Duration time	Pressure
Pre-freezing	-45℃	10min	60min	—
Primary Drying	-45℃	10min	60min	0.01mbar
	-40℃	10min	600min	0.01mbar
	-35℃	10min	120min	0.01mbar
	-30℃	20min	60min	0.01mbar
	-20℃	20min	60min	0.01mbar
	-10℃	20min	60min	0.01mbar
	0℃	20min	60min	0.01mbar
Secondary Drying	5℃	10min	20min	0.01mbar 0.01mbar
	10℃	10min	20min	0.01mbar 0.01mbar
	15℃	10min	20min	0.01mbar
	20℃	10min	20min	0.01mbar
	25℃	10min	240min	0.01mbar

Notes

1. This product is for research use only.

2. Please operate with lab coats and disposable gloves, for your safety.

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