
Lyo-Ready Taqman Multiplex qPCR Mix
Components
Lyo-Ready TaqMan qPCR Mix
Storage Conditions
This product should be stored at -25~-15℃ away from light for 1 year.
Instructions
1.Reaction system
Components |
Volume (μL) |
Final concentration |
Enzyme Mix |
20 |
- |
Primer Primer (10μM) |
x |
0.1-0.5μM |
Probe Primer (10μM) |
x |
50-250nM |
DNA Tamplate |
1-25 |
- |
dd H2O |
up to 25 |
- |
2.Reaction program
Cycle step |
Temp. |
Time |
Cycles |
Initial denaturation |
95℃ |
5mins |
1 |
Amplification reaction |
95℃ |
15sec |
45 |
Annealing/Extension |
60℃ |
30sec |
Notes: In special cases the annealing/extension temperature can be adjusted according to the primer Tm value, 60°C is recommended.
Components |
Volume (μL) |
Final concentration |
Lyo-ready Taqman qPCR mix
|
20 |
1X |
-50℃
|
x |
0.1-0.5μM |
-50℃ |
x |
50-250nM |
Total Volume |
21-25 |
- |
Lyophilization Process
Procedure |
Temp. |
Set time |
Duration time |
Pressure |
Pre-freezing |
-45℃ |
10min |
60min |
— |
Primary Drying |
-45℃ |
10min |
60min |
0.01mbar |
-40℃ |
10min |
600min |
0.01mbar |
|
-35℃ |
10min |
120min |
0.01mbar |
|
-30℃ |
20min |
60min |
0.01mbar |
|
-20℃ |
20min |
60min |
0.01mbar |
|
-10℃ |
20min |
60min |
0.01mbar |
|
0℃ |
20min |
60min |
0.01mbar |
|
Secondary Drying |
5℃ |
10min |
20min |
0.01mbar 0.01mbar |
10℃ |
10min |
20min |
||
15℃ |
10min |
20min |
0.01mbar |
|
20℃ |
10min |
20min |
0.01mbar |
|
25℃ |
10min |
240min |
0.01mbar |
Notes
1. This product is for research use only.
2. Please operate with lab coats and disposable gloves, for your safety.
Components
Lyo-Ready TaqMan qPCR Mix
Storage Conditions
This product should be stored at -25~-15℃ away from light for 1 year.
Instructions
1.Reaction system
Components |
Volume (μL) |
Final concentration |
Enzyme Mix |
20 |
- |
Primer Primer (10μM) |
x |
0.1-0.5μM |
Probe Primer (10μM) |
x |
50-250nM |
DNA Tamplate |
1-25 |
- |
dd H2O |
up to 25 |
- |
2.Reaction program
Cycle step |
Temp. |
Time |
Cycles |
Initial denaturation |
95℃ |
5mins |
1 |
Amplification reaction |
95℃ |
15sec |
45 |
Annealing/Extension |
60℃ |
30sec |
Notes: In special cases the annealing/extension temperature can be adjusted according to the primer Tm value, 60°C is recommended.
Components |
Volume (μL) |
Final concentration |
Lyo-ready Taqman qPCR mix
|
20 |
1X |
-50℃
|
x |
0.1-0.5μM |
-50℃ |
x |
50-250nM |
Total Volume |
21-25 |
- |
Lyophilization Process
Procedure |
Temp. |
Set time |
Duration time |
Pressure |
Pre-freezing |
-45℃ |
10min |
60min |
— |
Primary Drying |
-45℃ |
10min |
60min |
0.01mbar |
-40℃ |
10min |
600min |
0.01mbar |
|
-35℃ |
10min |
120min |
0.01mbar |
|
-30℃ |
20min |
60min |
0.01mbar |
|
-20℃ |
20min |
60min |
0.01mbar |
|
-10℃ |
20min |
60min |
0.01mbar |
|
0℃ |
20min |
60min |
0.01mbar |
|
Secondary Drying |
5℃ |
10min |
20min |
0.01mbar 0.01mbar |
10℃ |
10min |
20min |
||
15℃ |
10min |
20min |
0.01mbar |
|
20℃ |
10min |
20min |
0.01mbar |
|
25℃ |
10min |
240min |
0.01mbar |
Notes
1. This product is for research use only.
2. Please operate with lab coats and disposable gloves, for your safety.