Taqman Multiplex qPCR Premix
Cat No: HCB5061B
Taqman Multiplex qPCR Premix master mix is a new generation antibody modified Taq DNA Polymerase fluroescence quantitative qPCR can be used in lyophilization reagent. It is suitable for FAM/HEX/TET/ JOE/ROX etc. Double-labelled probes (also suitable for Molecular Beacon probes). The reagent contains hotstart Fast Taq DNA Polymerase, dNTP etc. Component which can be free-dried directly on the machine. The product does not contain ROX-corrected dyes and is suitable for various quantitative PCR models.
Components
Lyo-Ready TaqMan qPCR Mix
Storage Conditions
This product should be stored at -25~-15℃ away from light for 1 year.
Instructions
1.Reaction system
|
Components |
Volume (μL) |
Final concentration |
|
Enzyme Mix |
20 |
- |
|
Primer Primer (10μM) |
x |
0.1-0.5μM |
|
Probe Primer (10μM) |
x |
50-250nM |
|
DNA Tamplate |
1-25 |
- |
|
dd H2O |
up to 25 |
- |
2.Reaction program
|
Cycle step |
Temp. |
Time |
Cycles |
|
Initial denaturation |
95℃ |
5mins |
1 |
|
Amplification reaction |
95℃ |
15sec |
45 |
|
Annealing/Extension |
60℃ |
30sec |
Notes: In special cases the annealing/extension temperature can be adjusted according to the primer Tm value, 60°C is recommended.
Lyophilization Process
|
Procedure |
Temp. |
Set time |
Duration time |
Pressure |
|
Pre-freezing |
-45℃ |
10min |
60min |
— |
|
Primary Drying |
-45℃ |
10min |
60min |
0.01mbar |
|
-40℃ |
10min |
600min |
0.01mbar |
|
|
-35℃ |
10min |
120min |
0.01mbar |
|
|
-30℃ |
20min |
60min |
0.01mbar |
|
|
-20℃ |
20min |
60min |
0.01mbar |
|
|
-10℃ |
20min |
60min |
0.01mbar |
|
|
0℃ |
20min |
60min |
0.01mbar |
|
|
Secondary Drying |
5℃ |
10min |
20min |
0.01mbar 0.01mbar |
|
10℃ |
10min |
20min |
||
|
15℃ |
10min |
20min |
0.01mbar |
|
|
20℃ |
10min |
20min |
0.01mbar |
|
|
25℃ |
10min |
240min |
0.01mbar |
Notes
1. This product is for research use only.
2. Please operate with lab coats and disposable gloves, for your safety.
