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Uracil DNA Glycoylase (Glycerol-free) Featured Image
  • Uracil DNA Glycoylase (Glycerol-free)
  • Uracil DNA Glycoylase (Glycerol-free)

Uracil DNA Glycoylase (Glycerol-free)


CAS no.: 39369-21-7  EC no.: 3.2.2.27

Package: 100U, 500U,5KU,10KU

Product Description

Description

Thermosensitive UDG (uracil-DNA glycosylase) can catalyze the hydrolysis of the uracil base of the DNA chain containing uracil and the N-glycosidic bond of the sugar-phosphate backbone to release free uracil. Compared with ordinary UDG enzymes, thermosensitive UDG enzymes avoid the possible residual activity of conventional UDG enzymes after inactivation, which may degrade dU-containing amplification products at room temperature. This product works at room temperature and is temperature sensitive and prone to inactivation.

Chemical Structure

Chemical Structure7

Specification

Enzyme Glycosylase
Compatible Buffer Storage Buffer
Heat Inactivation 50°C, 10 min
Unit Definition One unit (U) is defined as the amount of enzyme required to catalyze the hydrolysis of 1 μg dU-containing dsDNA in 30 minutes at 25°C.

Applications

Remove dU-containing PCR product aerosol contamination.

Removal of uracil bases from single-stranded or double-stranded DNA

Shipping and Storage

Transportation: Ice packs

Storage Conditions: Store at  -15℃ ~ -25℃

Shief life:  1years


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