Hs Taq DNA Polymerase(Glycerol-free)

CAS no.: 9012-90-2 EC no.: 2.7.7.7

Package: 1000U 5000U 50000U

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Product Description

Advantages

Taq enzyme activity can be completely released by heating at 95°C for 30 s

High amplification sensitivity and specificity

Compatible with various PCR/qPCR systems

Description

Hot start Taq DNA Polymerase(Glycerol-free) is an antibody hot-start Taq DNA polymerase designed for developing lyophilized assays. Taq DNA polymerase is a hot-start Taq enzyme obtained by mixing Taq antibody and Taq DNA polymerase in a certain ratio. Based on the thermal stability of Taq antibody, Taq DNA polymerase can still maintain strict sealing at 55°C, so that non-specific amplification can be suppressed to a low degree during the sample mixing and system heating stages. When the reaction is kept at 95°C for more than 30 sec, the Taq antibody is completely inactivated, and the Taq enzyme activity is completely released, which ensures that the PCR system has high amplification sensitivity and specificity.

Chemical structure

Specification

Test items	Results
Protein Purity	≥95%
Blocking Effect	≥99%
Exonuclease Activity	Not detected
Nickase Activity	Not detected
Rnase Activity	Not detected
Amplification Sensitivity	Pass

Applications

Hs Taq DNA Polymerase Glycerol Free is developed for applications involving automation and freeze drying. Its Glycerol free formulation is well suited for automated routine PCR applications, or where accurate pipetting of small volumes is crucial.

What Glycerol Does

Glycerol is normally a major part of the storage buffer for enzymes, and acts as a cryoprotectant. Glycerol disrupts the water structure and makes the buffer more cell like, thus stabilising the polymerase. Glycerol is a highly viscous liquid and is therefore difficult and time-consuming to pipet accurately, especially in smaller volumes. As a consequence, pipetting glycerol in fast robot-aided automation processes is almost an unsolvable challenge. Furthermore, the presence of glycerol in the enzyme buffer makes freeze drying impossible.