One Step RT-qPCR Probe Kit (FOR FAST)
Description
One Step RT-qPCR Probe Kit (FOR FAST) is a probe-based RT-qPCR fast detection kit suitable for single-plex or multi-plex quantitative PCR using RNA as a template (such as RNA virus). This product uses a new generation of antibody-modified Taq DNA Polymerase and one-step dedicated Reverse Transcriptase, with an optimized buffer for rapid amplification, which has faster amplification speed, higher amplification efficiency and specificity. It supports balanced amplification in both single-plex and multi-plex of low and high concentration samples in a short time.
Reaction Process
Components
|
Components |
200rxns |
1,000rns |
10,000 rxns |
|
RNase-free ddH2O |
3*1ml |
15ml |
150ml |
|
5*one step mix |
800μl |
4*1ml |
40ml |
|
One step enzyme mix |
200μl |
1ml |
10ml |
|
50* ROX reference Dye 1 |
80μl |
400μl |
4*1ml |
|
50* ROX reference Dye 2 |
80μl |
400μl |
4*1ml |
a. One-Step Buffer includes dNTP Mix and Mg2+.
b. Enzyme Mix mainly contains reverse
transcriptase, Hot Start Taq DNA polymerase (antibody modification) and RNase inhibitor.
c. Used to correct the error of fluorescene sinals between diferent wells.
c. ROX: You need to choose the calibration according to the model of the testing instrument.
Applications
Detection of pathogens
diagnostics and research of tumor
Animal disease detection
Early diagnositics of hereditary diseases
Detection of pathogenic microorganisms in food
Shipping and Storage
Transportation: Ice packs
Storage Conditions: Store at -30 ~ -15℃.
Shief life: 1 years
