High Yield T7 RNA Poymerase
T7 RNA Polymerase is Isolated from E.coli carrying a plasmid which contains the T7 RNA Polymerase gene. T7 RNA Polymerase is a DNA-dependent RNA polymerase that is highly specific for the T7 phage promoters.
One unit is defined as the amount of enzyme that will incorporate 1 nmol ATP into acid-insoluble material in a total reaction volume of 50 μl in 1 hour at 37°C in 1X RNA Polymerase Reaction Buffer.
|Promoter Specificity||Nonspecific product≤1.5%|
|E.coli gDNA||≤0.1 pg/50U|
Transportation and storage
Transportation: Dry ice
Storage: All components should be stored at -25～-15°C
Recommended re-test Life: 2 year