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Proteinase K ( Lyophilized powder for NGS) Featured Image
  • Proteinase K ( Lyophilized powder for NGS)

Proteinase K ( Lyophilized powder for NGS)


Proteinase K ( lyophilized powder for NGS)

CAS no.: 39450-01-6

EC no.: 3.4.21.64

Package: 1g, 10g, 100g

Product Detail

Advantages

● Higher stability and enzyme activity based on directed evolution technologies

● Tolerant of Guanidine salt

● RNase free, DNase free and Nickase free

● Activity≥40U/mg

Chemical structure

Chemical structure

Description

NGS Protease K is a serine protease with high enzyme activity and wide substrate specificity. The enzyme preferentially decomposes ester bonds and peptide bonds adjacent to the  C-terminal of hydrophobic amino acids, sulfur-containing amino acids and aromatic amino acids. So, it is often used to degrade proteins into short peptides. NGS Protease K is a typical serine protease with the Asp39-His69-Ser224 catalytic triad which is unique to serine proteases, and the catalytic center is surrounded by tow Ca 2+ binding sites for stabilization, maintaining high enzyme activity under a wider range of conditions.

Specification

Test items

Specifications

Description

White to off white amorphous powder, Lyophilied

Activity

≥40U/mg

Solubility(50mg Powder/mL)

Clear

RNase 

None detected

DNase

None detected

Nickase

None detected

DNA

5pg/mg, solid

Bioburden

≤50 CFU/g, solid

Applications

Genetic diagnostic kit;

RNA and DNA extraction kits;

Extraction of non-protein components from tissues,degradation of protein impurities, such as;

DNA vaccines and preparation of heparin;

Preparation of chromosome DNA by pulsed electrophoresis;  

Western blot;

Enzymatic glycosylated albumin reagents in vitro diagnostics

Shipping and Storage

Shipping: Ambient

Storage Conditions:  Store at -20℃(Long term)/ 2-8℃( Short term)

Recommended re-test date: 2Years


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