
Proteinase K ( Lyophilized powder for NGS)
Advantages
● Higher stability and enzyme activity based on directed evolution technologies
● Tolerant of Guanidine salt
● RNase free, DNase free and Nickase free
● Activity≥40U/mg
Chemical structure

Description
NGS Protease K is a serine protease with high enzyme activity and wide substrate specificity. The enzyme preferentially decomposes ester bonds and peptide bonds adjacent to the C-terminal of hydrophobic amino acids, sulfur-containing amino acids and aromatic amino acids. So, it is often used to degrade proteins into short peptides. NGS Protease K is a typical serine protease with the Asp39-His69-Ser224 catalytic triad which is unique to serine proteases, and the catalytic center is surrounded by tow Ca 2+ binding sites for stabilization, maintaining high enzyme activity under a wider range of conditions.
Specification
Test items |
Specifications |
Description |
White to off white amorphous powder, Lyophilied |
Activity |
≥40U/mg |
Solubility(50mg Powder/mL) |
Clear |
RNase |
None detected |
DNase |
None detected |
Nickase |
None detected |
DNA |
5pg/mg, solid |
Bioburden |
≤50 CFU/g, solid |
Applications
Genetic diagnostic kit;
RNA and DNA extraction kits;
Extraction of non-protein components from tissues,degradation of protein impurities, such as;
DNA vaccines and preparation of heparin;
Preparation of chromosome DNA by pulsed electrophoresis;
Western blot;
Enzymatic glycosylated albumin reagents in vitro diagnostics
Shipping and Storage
Shipping: Ambient
Storage Conditions: Store at -20℃(Long term)/ 2-8℃( Short term)
Recommended re-test date: 2Years