Alkaline Phosphatase(ALP)–Biochemical diagnostics Featured Image
  • Alkaline Phosphatase(ALP)–Biochemical diagnostics
  • Alkaline Phosphatase(ALP)–Biochemical diagnostics

Alkaline Phosphatase(ALP)

Cas No.:9001-78-9

Purity.: 90%

Package: 100μL, 500μL, 10mL, 100mL,1000mL

Product Description


Alkaline Phosphatase is derived from a recombinant E. coli strain that carries the TAB5 gene. The enzyme catalyzes the dephosphorylation of 5´ and 3´ ends of DNA and RNA phosphomonoesters. Also, it hydrolyses ribose, as well as deoxyribonucleoside triphosphates (NTPs and dNTPs). TAB5 Alkaline Phosphatase acts on 5´ protruding, 5´ recessed and blunt ends. The Phosphatase can be used in many molecular biology applications, such as cloning or probe end labeling to remove the phosphorylated ends of DNA or RNA. In cloning experiments, dephosphorylation prevents the linearized plasmid DNA from self-ligation. It can also degrade unincorporated dNTPs in PCR reactions to prepare a template for DNA sequencing. The enzyme is completely and irreversibly inactivated by heating at 70°C for 5 minutes, thereby making removal of the phosphatase prior to ligation or end labeling unnecessary.

Chemical Structure



Test Items Specifications
Enzyme Activity 5U/μL
Purity ≥ 95%
Endonuclease Activity Not detectable
Exonuclease Activity Not detectable
Nicking Activity Not detectable
RNase Activity Not detectable
E.coli DNA ≤1copy/5U
Endotoxin LAL-Test, ≤ 10EU/mg

Transportation and storage

Transportation: Ice packs

Storage: Store at -25~-15°C(avoid repeated freezing and thawing)

Recommended re-test Life: 2 year

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