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  • T4 RNA ligase

T4 RNA ligase

Cat No:HCP1016A

Package: 100μL,500μL

Product Description


This product is an ATP-dependent T4 RNA ligase (T4 RNA Ligase I) recombinantly expressed by E. coli. This enzyme catalyzes the formation of phosphodiester bonds between oligonucleotides, single-stranded RNA and DNA intermolecular/ intra-molecular 5'-PO4 and 3'-OH.

Unit Definition

One unit is defined as the amount of enzyme required to convert 1 nanomole of 5´-[³²P] rA16 into a phosphatase-resistant form in 30 minutes at 37°C.


Test Items Specifications
Enzyme Activity 10 U/μL
Protein Activity ≥ 95%
Nickase Not detectable
RNase Activity Not detectable
Ecoli gDNA ≤1copy/50U
Endotoxin LCL-Test,≤10EU/mg

Transportation and storage

Transportation: Dry ice

Storage: Store at -25~-15°C(avoid repeated freezing and thawing)

Recommended re-test Life: 2 year

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